Toxicity Effect of Bromoacetic Acid on MCF7 Breast Cancer Cell Line and Analysis of Expression of Apoptosis-Associated Genes

نویسندگان

Bahman Jahromi, Enayatollah Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

Jafaarnejad, Leila Msc Student, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

Vahdani, Mina Msc Student, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

Zolghadri, Samaneh Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

چکیده مقاله:

Introduction: Cancer, the uncontrolled division of cells, occurs because of environmental factors and genetic disorders. Breast cancer is the most common cancer and the second leading cause of cancer death in women. Four categories of key genes, including oncogenes, tumor suppressor genes, repairing genes, and programmed– cell death genes, contribute to cancer development. Bromoacetic acid is a chemical compound that is produced both artificially and by bacterial fermentation. Methods: To investigate the effectiveness of bromoacetic acid in inhibiting the MCF7 cell line proliferation, the MTT assay was done and the expression of genes responsible for the regulation of apoptosis, including BAK, CASP3, CASP8, and BIM, was measured after 24, 48, and 72 hours of cell treatment with 2.5 µg/ml of bromoacetic acid using real–time PCR. Results: The results of the gene expression assays showed that bromoacetic acid treatment increased the expression of key genes BAK, CASP3, and, CASP8. However, the expression of BIM decreased at all three time points compared with controls. Conclusion: bromoacetic acid can induce cell death via intrinsic and extrinsic apoptosis pathways.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Anticancer Effect of Bovine Lactoferrin on Breast Cancer Cell Line MCF7 and the Evaluation of Bax and Bak Genes Expression Involved in Apoptosis

متن کامل

histological evaluation of the effect of three medicaments; trichloracetic acid, formocresol and mineral trioxide aggregate on pulpotomized teeth of dogs

چکیده ندارد.

15 صفحه اول

the effect of explicit teaching of metacognitive vocabulary learning strategies on recall and retention of idioms

چکیده ندارد.

15 صفحه اول

effect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars

این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...

Evaluating the Toxicity of Doxorubicin-Silk Fibroin Nanoparticles and Its Effect on P53 Gene Expression in Breast Cancer Cell Line

Introduction: The use of drug delivery systems can increase the effectiveness of chemotherapy and reduce its side effects in the treatment of breast cancer. This study aimed to evaluate the effect of doxorubicin-containing silk fibroin nanoparticles (NF-DOX) on P53 gene expression in breast cancer cell lines and to measure its toxicity in vitro. Methods: NF-DOX was synthesized and characterize...

متن کامل